Abstract
In this review, factors that contribute to enhancing the stability of immobilized enzyme membranes have been indicated, and the solutions to each factor, based on examples, are discussed. The factors are divided into two categories: one is dependent on the improvement of enzyme properties, and the other, on the development of supporting materials. Improvement of an enzyme itself would effectively improve its properties. However, some novel materials or novel preparation methods are required for improving the properties of supporting materials. Examples have been provided principally aimed at improvements in membrane stability.
Highlights
There has been growing interest concerning immobilized enzyme membranes in the last two decades [1]
If it is considered that the properties of the immobilized enzyme membranes consist of the combination of the properties of enzymes and supporting materials, heat tolerance would be mainly dependent on the enzyme properties
Polyion complex membranes would be stable materials for the prevention of fouling, One of the candidate materials is can cellulose, which is known and the enzyme-immobilized membranes be used for a long period.to be stable against chemical reagents [123,124] because it can only be reacted with strong acids, bases and highly reactive chemicals
Summary
There has been growing interest concerning immobilized enzyme membranes in the last two decades [1]. Immobilized enzymes can be applied to biosensing systems and to bioreactors, medical analysis, and biofuel cells [2,3,4,5,6,7], and the demands for high-performance immobilized-enzyme membranes have been increasing. Development of immobilized enzyme membranes are expected to advance performances of analyzers and bioreactors, and to enlarge their coverage areas such as enzyme-based fuel cells. In these developments, one demand is for the improvement of immobilized enzyme stability.
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