Abstract
Abstract Freeze substitution is a crucial technique in electron microscopy, enabling the preservation of biological specimen ultrastructure. However, existing tools are primarily designed for 3 mm carriers, limiting their compatibility with larger carriers such as CryoCapsules or 6 mm carriers. In this study, we developed a novel freeze substitution tool, simply named the FS-basket, to address the challenges associated with embedding samples vitrified in larger carriers. The tool comprises a mesh with pore sizes smaller than the sample chunks, deposited below the carrier to maintain a tight seal while allowing for easy fluid exchange.
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