Abstract
Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) are widely used to determine the size of biological nanoparticles in liquid. In both cases, one first measures the nanoparticle diffusion coefficient and then converts it to the nanoparticle radius via the Stokes-Einstein relation. This relation is based on the no-slip boundary condition. Now, there is evidence that this condition can be violated in biologically relevant cases (e.g., for vesicles) and that in such situations the partial-slip boundary condition is more suitable. I show (i) how the latter condition can be employed in the context of DLS and NTA and (ii) that the use of the former condition may result in underestimation of the nanoparticle radius by about 10 nm compared with the nominal one.
Highlights
Biological nanoparticles, e.g., lipid vesicles, micelles, bicelles, virions, and lipid or colloidal nanoparticles employed for drug or RNA delivery, are usually suspended in liquid, and the determination of their size is an important step in their studies and/or the use in applications
I show (i) how the latter condition can be employed in the context of Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) and (ii) that the use of the former condition may result in underestimation of the nanoparticle radius by about 10 nm compared with the nominal one
In general the measurement of size can be done by employing various techniques, practically, it is often performed by using DLS and/or NTA
Summary
Biological nanoparticles, e.g., lipid vesicles, micelles, bicelles, virions, and lipid or colloidal nanoparticles employed for drug or RNA delivery, are usually suspended in liquid, and the determination of their size is an important step in their studies and/or the use in applications. Abstract Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) are widely used to determine the size of biological nanoparticles in liquid. I show (i) how the latter condition can be employed in the context of DLS and NTA and (ii) that the use of the former condition may result in underestimation of the nanoparticle radius by about 10 nm compared with the nominal one.
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