Abstract
The paper shows how polysiloxane particles encapsulating fluorophores can besuccessfully used to detect biotin–streptavidin binding by two types of technique. Afterfunctionalization of the particles by streptavidin, the fixation of the biomolecule can indeedbe detected by a shift of the localized surface plasmon resonance of the biotinylated golddots used as substrate and by the luminescence of the fluorophores evidenced byscanning near-field optical microscopy. The development of particles allowingsuch a double detection opens a route for increasing the reliability of biologicaldetection and for multi-labelling strategies crossing both detection principles.
Published Version
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