Abstract
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) formation and phagocytosis. Therefore, fresh porcine whole blood was compared to whole blood stored for 24 h in the dark at room temperature. Different cell parameters in whole blood and in isolated neutrophils were analyzed. The following parameters were analyzed: cell count, band and segmented neutrophil count, viability, cholesterol content, release of free DNA as a marker for cell death, phagocytic activity in whole blood and in isolated neutrophils, the transmigration rate of neutrophils to IL8 stimulus, the production of reactive oxygen species (ROS), and the formation of NETs. It was observed that the number of isolated neutrophils decreased over time, indicating cell death occurs during 24 h of blood storage. However, the surviving neutrophils isolated from stored blood reacted comparably or even showed enhanced antimicrobial activity in the case of phagocytosis of Streptococcus (S.) suis, ROS production, and transmigration. The slightly altered cholesterol level of the harvested neutrophils in stored blood when compared to fresh blood partially explains some of the detected differences.
Highlights
Neutrophils are one of the most abundant leukocytes in blood circulation
IL17 was determined as a cytokine that is involved the recruitment of neutrophils [16] and neutrophils can produce IL17 on their own to enhance the recruitment of more cells [17]
We focused on isolated neutrophils and examined whether the same antimicrobial activity tendency was detected as shown for the whole blood assay (Figure 8)
Summary
Neutrophils are one of the most abundant leukocytes in blood circulation. In humans around50–70% [1] and in pigs around 15–72% [2,3,4] circulating leukocytes are neutrophils. Biomedicines 2020, 8, 278 different antimicrobial components, for example, myeloperoxidase, neutrophil elastase or antimicrobial peptides [6,7]. These different components can kill a wide variety of microorganisms. Neutrophils can release these antimicrobial components from vesicles/granules to the extracellular space. This process is called degranulation of the neutrophil [5]. Another extracellular mechanism for controlling pathogens is the release of neutrophil extracellular traps (NETs) [8]. Pathogens can be killed or at least immobilized inside these
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
