How does silicon alleviate Cd-induced phytotoxicity in barley, Hordeum vulgare L.?

Abstract

Toxic heavy metal accumulation in edible plants has become a problem worth worrying about for human health. Cadmium is one of the most toxic metals presenting high bioavailability in the environment. The main route of transfer of Cd to humans is the consumption of contaminated food which suggests that reducing of Cd absorption by plants could reduce this risk. In this context, it was suggested that silicon supply would be able to limit the transfer of Cd to the plants. Thus, this work evaluated the effects of 0.5 mM Si on Cd absorption and accumulation in barley (Hordeum vulgare L.). Plants were grown hydroponically for 21 days in the presence of 0 and 15 μM Cd2+ combined or not with 0.5 mM Si. Analyses were related to growth and photosynthesis parameters, Cd accumulation in organs and Cd subcellular distribution in the shoots. Results showed that, under Cd alone, plants showed severe toxicity symptoms as chlorosis and necrosis and produced significantly less biomass as compared to control. 0.5 mM Si in the medium culture significantly reduced Cd-induced toxicity by mitigating symptoms and restoring growth, photosynthesis, and nutrition. Si also induced a significant reduction of Cd concentration in plants and changed its sub-cellular compartmentalization by enhancing fixation to cell walls and reducing the Cd concentration in the cytoplasmic and organelles fractions. These data suggest that the application of Si could significantly increase Cd tolerance and reduce the risk of the Cd accumulation in edible plants.