How does population genetic diversity change over time? An experimental seed bank study of Atriplex tatarica (Chenopodiaceae)

Abstract

Atriplex tatarica is an annual, early successional, facultative halophilic species of frequently disturbed human-made habitats in Central and Eastern Europe. We investigated to what extent the plants grown from seeds extracted from soil seed bank differed genetically to mature aboveground plants in experimental populations of A. tatarica over two successive years. At each of five plots 50 aboveground plants and 50 plants extracted from seeds stored in soil were assayed for allozyme analysis in 2003 and 2004. At the start of experiment, we introduced 1000 seeds of the study species into each of five experimental plots. While the species dominated in all of the experimental plots in the first year, the second year A. tatarica coverage decreased dramatically. Overall allele frequencies of soil seeds and mature plants showed significant differences between life history stages in both years, but not within years in soil seeds as well as mature plants stages. While mature plants showed a significantly greater amount of single and multilocus heterozygosity in both consecutive years, comparison between years did not yield any significant differences. In the same way, despite a relatively large seed bank the species population genetic parameters, i.e. allelic richness ( A), observed heterozygosity ( H o), gene diversity ( H s), inbreeding coefficient ( F IS) and fixation index ( F ST), did not change over years between as well as within life history stages. The soil seeds and mature plants significantly differed in H o, H s and F IS, while the A and F ST were not significantly different between life history stages.