How does multiannual plastic mulching in strawberry cultivation influence soil fungi and mycotoxin occurrence in soil?

Abstract

The production of mycotoxins is often interpreted as fungal response to cope with unfavorable growth conditions induced by toxic substances, environmental and biological factors. Soil covers influence soil environment, which consequently can change the abundance and composition of microbial communities. We investigated how plastic coverage (PC) influence soil fungi and mycotoxin occurrence (deoxynivalenol, nivalenol and zearalenone) compared to the traditional straw coverage (SC) in dependence of soil depth and time in a 3-year field experiment in strawberry cultivation. In total, 300 soil samples, resulting from two treatments, three soil layers, and ten sampling dates (n = 5), were analyzed for mycotoxins and ergosterol (proxy for soil fungal biomass) with liquid chromatography high resolution mass spectrometry and high-performance liquid chromatography with UV-detection, respectively. The modified microclimate under PC had no significant influence on fungal biomass, whereas SC promoted fungal biomass in the topsoil due to C-input. Mycotoxins were detected under both cover types in concentrations between 0.3 and 21.8 µg kg−1, mainly during strawberry establishment period and after fungicide application. Deoxynivalenol had the highest detection frequency with 26.3% (nivalenol: 8.3%, zearalenone: 8.7%). This study confirmed the in situ production of mycotoxins in soil, which seems mainly triggered by field treatment (fungicide application) and plant growth stage (establishment period) rather than on mulching type. Further investigations are necessary to better understand the influence of different agricultural practices and soil types on the production and fate of mycotoxins.