How does maturity stage affect seeds metabolome via UPLC/MS based molecular networking and chemometrics and in relation to antioxidant effect? A case study in 4 major cereals and legumes.

Abstract

Legumes and cereals as staple food are typically consumed at mature stage, though also consumed at earlier stages. UPLC/MS based molecular networking and chemometrics were employed for the first time to address metabolome composition heterogeneity amongst seeds in the context of their maturity stages. The study included 4 major cereal and leguminous seeds of different species, and cultivars i.e., Triticum aestivum, Hordeum vulgare, Vicia faba and Cicer arietinum. 146 Metabolites from various classes were identified of which several are first time to be reported. Supervised OPLS model of all datasets revealed that sugars and oxylipids were dominant in mature and immature seeds, respectively. DPPH and FRAP assays were assessed for differential secondary metabolites' correlation. Results were attributed to flavonoids, oxylipids, and amino acids/peptides. Mature barley seeds possessed the strongest antioxidant activity among examined seeds. This study provides novel insights on seeds' maturation process in context to holistic metabolic changes.