Abstract
LGC validated the PowerPlex® ESI17 Fast system (ESI17F), (Promega) in July 2014. As part of the validation a characterisation study was performed to assess kit performance at different DNA inputs.Following implementation of the ESI17F system, reports of lower than expected peak heights and heterozygous imbalance being present in optimal input (500pg) samples were made. A review of profile quality for single source samples with optimal DNA input was carried out. A number of variables were assessed to see if there was a connection with the observed reduced peak height and heterozygous events. Sample degradation was observed to be the major cause of reduced peak heights and heterozygous imbalance in samples with optimal DNA input.Evidence of DNA degradation was observed in ∼50% of samples reviewed. Although the ESI17F system provides smaller amplicons for the loci that are required to load to the UK National DNA database; the larger number of loci present within the kit results in a larger number of large amplicons that are more likely to be affected by DNA degradation.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Forensic Science International: Genetics Supplement Series
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
