How clean is your ice machine? Revealing microbial amplification and presence of opportunistic pathogens in hospital ice-water machines

Abstract

Ice machines in healthcare facilities have been suspected and even linked to outbreaks and pseudo-outbreaks. Guidelines exist for maintenance of these devices but there is no clear independent infection control standard, and little is known on their microbial contamination. To evaluate the microbial contamination, amplification, and presence of opportunistic pathogens in ice-water machines in a healthcare facility. Concentrations of general microbial indicators (heterotrophic plate counts (HPC), total and intact cells), faecal indicators (enterococci) and opportunistic pathogens (Pseudomonas aeruginosa, nontuberculous mycobacteria (NTM), Candida spp.) were measured in 36 ice-water machines on patient wards of a 772-bed hospital. Profile sampling was performed on 5 ice-water machines and adjacent faucets to identify sites of microbial proliferation. Candida spp. were found in half of ice-water samples while enterococci and P. aeruginosa were present in 6 and 11 drain inlets respectively. NTM were measured in all ice-water samples and 35/36 biofilms. Pre-filters and ice machines are sites for additional amplification: NTM densities were on average 1.3 log higher in water of ice machine flushed 5 min compared to flushed adjacent tap water. Ice machines design needs to be adapted to reduce microbial proliferation. The absence of correlation between HPC densities (current microbial indicators) and NTM concentrations suggests a need for cleaning efficiency indicators better correlated with opportunistic pathogens. Cleaning and disinfection guidelines of ice machines in healthcare facilities need to be improved, especially when ice is given to the most vulnerable patients, and NTM may be an efficiency indicator.