How APC/C–Cdc20 changes its substrate specificity in mitosis

Abstract

SummaryProgress through mitosis requires that the right protein be degraded at the right time. One ubiquitin ligase, the Anaphase Promoting Complex or Cyclosome (APC-C) targets most of the crucial mitotic regulators by changing its substrate specificity throughout mitosis. The Spindle Assembly Checkpoint (SAC) acts on the APC-C co-activator, Cdc20 to block the degradation of metaphase substrates, e.g.: Cyclin B1 and securin, but not others, e.g.: Cyclin A. How this is achieved is unclear. Here we show that Cdc20 binds to different sites on the APC-C depending on the SAC. Cdc20 requires APC3 and APC8 to bind and activate the APC-C when the SAC is satisfied, but only requires APC8 when the SAC is active. Moreover, APC10 is crucial for Cyclin B1 and securin but not Cyclin A destruction. We conclude that the SAC causes Cdc20 to bind to different sites on the APC-C and this alters APC-C substrate specificity.