Abstract
Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma.
Highlights
More than 100 human adenoviruses (AdV) have been identified.They have been phylogenetically divided into seven species (A to G) based on hemagglutination features, oncogenic potential in rodents, DNA homology, and genome organization [1,2]
In comparison to house dust mite (HDM)-provoked nasal epithelial cells (NAEPCs), we observed a significant increase of coxsackie andadenovirus receptor (CAR) and CD46 expression levels on NAEPCs of asthmatics as well as on NAEPCs of healthy donors
In comparison to HDM-provoked NAEPCs, we observed a significant increase of transduction efficiency of NAEPCs after HDM exposition (Figure 7a,b)
Summary
More than 100 human adenoviruses (AdV) (http://hadvwg.gmu.edu/) have been identified. They have been phylogenetically divided into seven species (A to G) based on hemagglutination features, oncogenic potential in rodents, DNA homology, and genome organization [1,2]. Human AdV have become increasingly important in recent years. They cause different clinical symptoms in a wide range of diseases, e.g., pneumonia, conjunctivitis, gastroenteritis, or myocarditis [5,6,7,8]. AdV have been causatively associated with pneumonia outbreaks in US-military bases [9]. Several AdV can be isolated from patients with lung infections [8], and here we addressed the question of whether this is associated with asthma exacerbation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
