Abstract

The extraction condition of crude polysaccharides from Codonopsis pilosula by hot water reflux method was studied and the results analyzed by kinetic model. Then, the extraction rate and maximum extraction temperature were determined. furthermore, the thermal and pH stability and the rheological properties of the crude polysaccharide were also studied. crude Codonopsis pilosula polysaccharide (CCPP) under different conditions was extracted by hot water reflux method, the content was determined by phenol-sulfuric acid and DNS method, and the kinetic model of the extraction process was established. The viscosity was determined under different solute concentration, salt concentration, rotation speed, pH, and temperature. The thermal stability and pH stability were determined; the protein content and sulfate ion content were determined by the coomassie brilliant blue method and barium sulfate turbidimetry method, respectively. The yield of CCPP by the hot water reflux method was 25.72%. At 50 oC, the maximum extraction concentration, the rate constant and the linear relationship between the rate constant and the time were obtained at different ratios of material to liquid. The extraction process of this experiment coincided with Fick`s second law, R2 was greater than 0.88 and with the increase of extraction time, the dissolution rate increased, but with the increase of the ratio of material to liquid, the dissolution rate was decreased. CCPP showed a loss of polysaccharide fluidity as temperature increased and was affected by pH change. The apparent viscosity was affected by a change in solute concentration, temperature, pH, rotation speed and salt and sucrose concentrations.

Highlights

  • Codonopsis pilosula is a medicinal plant of the family Campanulaceae that is predominantly found in Asia [1]

  • Polysaccharide yield and kinetic model In this experiment, 129 g C. pilosula was used and 33.18 g crude polysaccharide was obtained by hot water extraction and ethanol precipitation

  • The yield of crude polysaccharide extracted from C. pilosula by hot water reflux method was calculated to be 25.72%

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Summary

Introduction

Codonopsis pilosula is a medicinal plant of the family Campanulaceae that is predominantly found in Asia [1]. There are about 40 Codonopsis species that can be found in China [2], and among them, Codonopsis pilosula is the most popular. It has long been used in Chinese medicine as a natural herb and occasionally as a substitute of Panax ginseng [3]. The roots of Codonopsis pilosula have been reported to have been used in traditional Chinese medicine for the treatment of asthma, cough, and psychoneurosis [4,5] and there are reports that Codonopsis species contain phytochemicals such as polysaccharides, triterpenoids, alkaloids, flavones, polyacetylenes, etc some of which are responsible for its bioactivities [1,3]

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