Hot hypoxic flies: Whole-organism interactions between hypoxic and thermal stressors in Drosophila melanogaster

Abstract

The upper critical thermal maximum (CT max) of metazoans varies over a wide range, and its determinative factors, such as oxygen limitation, remain controversial. Induction of thermoprotective mechanisms after challenge by sublethal heat stress has been well documented in many organisms, including the model fly Drosophila melanogaster. Interestingly, however, other challenges—notably a period of anoxia—induce post-exposure thermoprotective effects in some organisms such as locusts and houseflies. Here I show, using thermolimit respirometry, that acute hypoxia during thermal stress significantly reduced the CT max of D. melanogaster, but only below an oxygen partial pressure of about 10 kPa (39.0±0.4 SE °C at 9.3 kPa vs. 36.0±0.2 SE °C at 3.5 kPa). Likewise, the scope for voluntary motor activity declined sharply below 10 kPa and was essentially eliminated at 2.3 kPa. Respiratory water loss increased highly significantly below about 10 kPa. The post-CT max release of a large quantity of CO 2 is shown to be independent of loss of spiracular control, but dependent at least in part on oxygen availability. The results are broadly in accord with Pörtner's oxygen limitation hypothesis, but suggest that acute oxygen limitation only becomes an important factor at partial pressures less than half of typical atmospheric levels.