Hot electron-induced electrochemiluminescence of polystyrene modified electrode for rutin determination

Abstract

• The spin-coating strategy is chosen to prepare the insulating layer, which is much simpler and controllable. • Polystyrene insulating layer can generation stable HECL. • Owing to the quenching effect on the SO 4 − radical, the present sensing platform was applied for the determination of rutin. For hot electron-induced electrochemiluminescence (HECL), developing facile and controllable insulating layer on the electrode surface is key to the effective electron tunneling. Herein, a polystyrene modified glassy carbon electrode based the spin-coating was proposed for HECL. The constructed interface was characterized by atomic force microscopy (AFM). And HECL performance was investigated in details using the system of Ru(bpy) 3 2+ and S 2 O 8 2− . The as-prepared film shows the stable and reproducible HECL performance and the HECL mechanism was discussed using ECL spectra and hydrated electron quenchers. Finally, on the basis of the inhibition of SO 4 − , the present polystyrene film was applied to the detection of rutin in the range of 0.1–1.3 μM with a detection limit of 90 nM (S/N = 3).