Abstract
Bacteriophage λ grown in auxotrophic met−, pro− or arg− strainsf of Escherichia coli K12 in the presence of the required amino acids show an efficiency of plating of approximately 1 on E. coli strains K12 and C. However, if met− cells are deprived of methionine during a portion of the latent period, the efficiency of plating of the progeny phage is lower on the host K12 than on strain C. Similar results are obtained with met− auxotrophs of strains B and K12(P1); deprivation of methionine during the latent period results in the production of phage with lower efficiency of plating on the host strain than on strain C. Such an effect is not observed following a similar starvation for proline or arginine. These results suggest that methionine is specifically required for the production of host specificity of DNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
