Host species and strain differences affect the ability of an HSV-1 ICP0 deletion mutant to establish latency and spontaneously reactivate in vivo

Abstract

HSV-1 latency and reactivation were studied in vivo by spontaneous and iontophoresis-induced ocular viral shedding in New Zealand rabbits, Balb/c and A/J mice latently infected with wild-type KOS, and dl × 3.1, a progeny ICP0 deletion mutant. The presence of trigeminal ganglionic latency was demonstrated by the in vitro methods of cocultivation and in situ hybridization. Although the efficiency of ganglionic latency was significantly less ( P < .0001) for dl × 3.1 than for KOS in both mice and rabbits, only dl × 3.1 shed spontaneously in the NZ rabbit. lontophoresis of adrenergic agents failed to induce reactivation and ocular viral shedding of KOS or dl × 3.1 in mice or rabbits. The establishment of latency and reactivation of KOS and dl × 3.1 was dependent on the host animal. We conclude that host factors as exemplified by host species and host strain differences significantly affected the ability of KOS and dl × 3.1 to establish latency, to reactivate, and to shed spontaneously. ICP0 expression was not required for the establishment or maintenance of latency, nor was it required for the reactivation of latent HSV-1. Furthermore, the biological activity of KOS and dl × 3.1 during latency in vivo did not correlate with latency studies based on in vitro methods.