Host range differentiation of spore‐positive and spore‐negative strain types of Frankia in stands of Alnus glutinosa and Alnus incana in Finland

Abstract

Host compatibility of different spore‐positive (Sp+)and spore‐negative (Sp−) strain types of Frankia from alder stands in Finland was studied in Modulation tests with hydrocultures of Alnus glutinosa (L.) Gaertner, A. incana (L.) Moench and A. nitida Endl. Root nodules and soil samples from stands of A. incana (Lammi forest and Hämeenlinna forest) were dominated by Sp + types of Frankia (coded AiSp+ and AiSp+ H. respectively), which caused effective root nodules in test plants of A. incana, but failed to induce nodules in A. nitida. In A. glutinosa Frankia strain types AiSp + and AiSp + H caused small, ineffective root nodules with sporangia (coded Ineff −), which were recognized by the absence or near absence of vesicles in the nodule tissue. Ineffective nodules without sporangia (coded Ineff −) were induced on A. glutinosa with soil samples collected at Lammi swamp. The spore‐negative strain type of Frankia was common in root nodules of A. glutinosa in Finland (Lammi swamp) and caused effective Sp− type root nodules (coded AgSp −) in hydrocultures of A. incana, A. glutinosa and A. nitida. A different Sp + strain type of Frankia. coded AgSp+ Finland, was occasionally found in stands of A. glutinosa. It was clearly distinguished from strain type AiSp + by the ability to produce effective nodules on both A. glutinosa and A. incana. The nodulation capacities of soil and nodule samples were calculated from the nodulation response in hydrocutlure and served as a measure for the population density of infective Frankia particles. Sp + nodules from both strain types had equal and high nodulation capacities with compatible host species. The nodulation capacities of Sp type root nodules from A. glutinosa were consistently low. High frequencies of Frankia AiSp+ and AiSp+ H were found in the soil environment of dominant AiSp + nodule populations on A. incana. The numbers of infective particles of this strain type were insignificant in the soil environment of nearby Sp − nodule populations on A. glutinosa and in the former field at Hämeen‐linna near the Sp+ nodule area in Hämeenlinna forest. Strain type AgSp− had low undulation capacity in the soil environment of both A. incana and A. glutinosa stands, Explanations for the strong associations between Frankia strain types AiSp+ and AiSp − H and A. incana and between strain type AgSp− and A. glutinosa are discussed in the light of host specificity and of some characteristics of population dynamics of both strain types. The possible need to adapt the concept of Frankia strain types Sp + and Sp − to strains with some variation in spore development was stressed by the low potentials of strain type AiSp + H to develop spores in symbioses with hydrocultures of A. incnna.