Host, pathogen, and environmental characteristics predict white-nose syndrome mortality in captive little brown myotis (Myotis lucifugus).

Joseph S Johnson,James W Mcmichael,Melissa B Meierhofer,Daniel W F Stern,Lauren E Sigler,Joseph A Kath,Deeann M Reeder,Allen Kurta,Shayne S Lumadue,Kenneth A Field,Harrison D Winters,Megan E Vodzak

doi:10.1371/journal.pone.0112502

Joseph S Johnson, James W Mcmichael + Show 10 more

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https://doi.org/10.1371/journal.pone.0112502

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Abstract

An estimated 5.7 million or more bats died in North America between 2006 and 2012 due to infection with the fungus Pseudogymnoascus destructans (Pd) that causes white-nose syndrome (WNS) during hibernation. The behavioral and physiological changes associated with hibernation leave bats vulnerable to WNS, but the persistence of bats within the contaminated regions of North America suggests that survival might vary predictably among individuals or in relation to environmental conditions. To investigate variables influencing WNS mortality, we conducted a captive study of 147 little brown myotis (Myotis lucifugus) inoculated with 0, 500, 5 000, 50 000, or 500 000 Pd conidia and hibernated for five months at either 4 or 10°C. We found that female bats were significantly more likely to survive hibernation, as were bats hibernated at 4°C, and bats with greater body condition at the start of hibernation. Although all bats inoculated with Pd exhibited shorter torpor bouts compared to controls, a characteristic of WNS, only bats inoculated with 500 conidia had significantly lower survival odds compared to controls. These data show that host and environmental characteristics are significant predictors of WNS mortality, and that exposure to up to 500 conidia is sufficient to cause a fatal infection. These results also illustrate a need to quantify dynamics of Pd exposure in free-ranging bats, as dynamics of WNS produced in captive studies inoculating bats with several hundred thousand conidia may differ from those in the wild.

Highlights

White-nose syndrome (WNS) is a fungal disease affecting hibernating bats, causing the death of an estimated 5.7–6.7 million bats since its initial discovery in North America in 2006 (USFWS 2012)
Large-scale mortality of bats has never been documented in Europe, 90% mortality of bats occurs in North American hibernacula after Pseudogymnoascus destructans (Pd) is introduced [14,15]
We found that WNS mortality is influenced by the level of Pd exposure, characteristics of the host, and the environment, and that several variables have interacting effects

Summary

Introduction

White-nose syndrome (WNS) is a fungal disease affecting hibernating bats, causing the death of an estimated 5.7–6.7 million bats since its initial discovery in North America in 2006 (USFWS 2012). WNS is caused by the cold-adapted fungus Pseudogymnoascus destructans (Pd), which invades the dermis and epidermis of bats during hibernation [1,2,3]. Large-scale mortality of bats has never been documented in Europe, 90% mortality of bats occurs in North American hibernacula after Pd is introduced [14,15]. Such high mortality rates have led to predictions of regional extinctions, not all bat species appear affected by the disease [15,16]

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