Abstract
Physalis minima is an herbaceous plant and inhabitant of the porous and organic matter containing soil of bunds in crop fields, wastelands, around the houses, and on the roadsides. S. rolfsii is soil borne and it can infect over 500 plant species of different families. It is of interest to study the pathogenesis of S. rolfsii on P. minima. The S. rolfsii isolated from P. minima (physr1) was characterized by morphology and sequence of Internal Transcribed Spacer (ITS) region. The population structure determination and phylogenetic analysis showed the isolate physr1 significantly differs from other isolates. The null hypothesis of equal evolutionary rate was rejected throughout the Maximum likelihood (ML) tree topology of different S. rolfsii ITS sequences. The site-specific mean (relative) evolutionary rate analysis showed that most of the sites (80.59 % sites) evolved at a slower rate than average. Finally, the result of Tajima's neutrality test indicated that the population of S. rolfsii has recently begun to expand and that's why the pathogen was infecting the new host P. minima and pose a serious threat of infecting several other cropped and non-cropped hosts.
Highlights
Physalis minima is an herbaceous plant and inhabitant of the porous and organic matter containing soil of bunds in crop fields, wastelands, around the houses, and on the roadsides
In addition to Physalis minima L. (Ground cherry plant – local name: ban tipariya systematic studies, molecular data have been used in conjunction - Bengal) is an herbaceous plant that belongs to the family with independent criteria such as pathogenicity, host range, solanaceae, and is commonly found on the bunds of the crop geographic origin, and vegetative compatibility groups (VCGs) to fields, wastelands, around the houses, on roadsides, etc., where examine the diversity among and within populations of the the soil is porous and rich in organic matter
Infected plants were taken from the field and isolations from field-grown plant yielded S. rolfsii that was identified on the basis of morphology
Summary
Physalis minima is an herbaceous plant and inhabitant of the porous and organic matter containing soil of bunds in crop fields, wastelands, around the houses, and on the roadsides. Though the isolate with other S. rolfsii isolated from different hosts on the fungus is seed and soil borne, soil borne inoculum is more basis of morphological, ITS sequence and studied the impact of important in causing infection and disease development. DNA extraction from S. rolfsii: Discs of 5 mm diameter S. rolfsii isolates were cut from periphery of an actively growing 7 days old culture on PDA and inoculated into 250 ml conical flask containing 50 ml of sterile potato dextrose broth.
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