Host dependent differential spread of Glomus intraradices on various Ri T-DNA transformed roots in vitro

Abstract

Carrots (Daucus carota) are used extensively for culturing arbuscular mycorrhizal fungi under the root organ culture system (ROC) in vitro. Four different cultivars of carrot and one of clover (Trifolium subterraneum) root cultures were used to investigate symbiotic events with Glomus intraradices when introduced to different host-transformed roots. The investigation was directed to study the state of mycorrhization, its pattern and variation, if any and, the differences in nutritional uptake of each AM-host symbiosis. The findings suggest the role of the host on the differential expression of G. intraradices with respect to colonization, spore production, intraradical and extramatrical spread of the fungus, nutritional kinetics of each host-fungus symbiosis, pattern of growth and differences in anatomical and morphological features of host roots.