Abstract
Temperature-sensitive mutants of SV40, ts-701 and ts-702, have been isolated after treatment of wild-type virus with nitrous acid and nitrosoguanidine, respectively. Although neither mutant produces infectious virus at the nonpermissive temperature, both can induce SV40 T and V antigens, virus DNA synthesis, and the stimulation of host cell DNA synthesis. Both are late mutants and appear to be in the same SV40 complementation group. Both mutants can also complement the replication of human adenovirus type 7 at the elevated temperature, as can another late mutant, pm-301, and an early mutant, tsA7. Therefore, the adenovirus complementation step appears to be prior to SV40 virus DNA synthesis. Since a BSC-1 clone in which SV40 infection stimulates host cell DNA synthesis allows the complementation of adenovirus type 7 by SV40 while parental BSC-1 cells neither support complementation of adenovirus type 7 by SV40 nor are stimulated to replicate their DNA after SV40 infection, the stimulation of host cell DNA synthesis appears to be critical to adenovirus enhancement. This stimulation appears to be specific since stimulation of DNA synthesis after nutrient deprivation does not induce the replication of human adenoviruses in simian cells.
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