Abstract
In this paper we present the first results of enzymatic activities in a reverse microemulsion medium based on a mixture of an anionic and a cationic surfactant, called catanionic microemulsion. The studied system is composed of sodium dodecyl sulfate (SDS)/dodecyltrimethylammonium bromide (DTAB)/n-hexanol/citrate buffer/n-dodecane, with high SDS/(SDS + DTAB) weight fractions. It turns out that the results are similar to those obtained in classical reverse microemulsions, except that the presence of DTAB exerts an inhibiting effect on the enzyme. Nevertheless, enzymatic superactivities are found even at a DTAB to total surfactant ratio of 15%, corresponding to 3% weight fraction of cationic surfactant in the microemulsion. The influence of pH and hexanol content on the enzymatic activities is also studied.
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