Hormone secretion and characteristics of estrous cycles after treatment of heifers with human chorionic gonadotropin or prostaglandin F2 alpha during corpus luteum formation.

Abstract

Fertility in cattle is related positively to concentrations of progesterone in blood during the estrous cycle preceding insemination. This study determined whether treatment of heifers with prostaglandin F2 alpha (PGF2 alpha) or human chorionic gonadotropin (hCG) during d 2 to 4 of an estrous cycle affected progesterone during that cycle and whether hormone secretion during the cycle and onset of subsequent estrus were related to progesterone secretion. Nine Holstein heifers were assigned to an experiment designed as a triplicate Latin square, and each heifer received each of three treatments during three consecutive estrous cycles. Treatments were: saline (control, 1 ml) on d 2, 3 and 4 after estrus; hCG, 1000 IU on d 2, 3 and 4; and PGF2 alpha, 25 mg on d 3 with repeated doses 12 and 24 h later. Progesterone throughout the estrous cycle was higher in heifers given hCG than in those given saline. Progesterone during the first week of the cycle was lower in heifers given PGF2 alpha than those given saline, but means for these two groups were similar thereafter. Number of peaks of 15-keto,13,14-dihydro-PGF2 alpha (PGFM) during 24 h after onset of luteolysis was lower in heifers given hCG than in those given saline or PGF2 alpha. Patterns of secretion of luteinizing hormone and estradiol at subsequent estrus were not affected by treatment. Temporal relationships among hormone secretion and onset of estrus were unaffected by treatment.