Abstract
Summary. Developmental changes in the response of mouse, rat and rabbit foetal liver cells and of rat foetal spleen cells to erythropoietin have been investigated in vitro.Liver cells from 101/2‐141/2‐day mouse foetuses and 12–13‐day rat foetuses show little haemoglobin synthesis in vitro but marked response to erythropoietin. This is followed, in the mouse, by a brief period in which the rate of synthesis on explantation is high and cannot be increased by erythropoietin. In the rat from 14 to 16 days the rate of synthesis on explantation increases progressively while the response to erythropoietin persists. Liver cells from later foetuses of both animals show variable haemoglobin synthesis and are not erythropoietin sensitive. 14–18‐day foetal rabbit liver cells and 18–19‐day foetal rat spleen cells are also sensitive to erythropoietin. It is suggested that development of sensitive stem cell populations precedes erythropoietin production in vivo so that critical population numbers are reached which enable feedback controls to be established.Isotopically labelled haenioglobins produced in vitro by rat and mouse foetal liver cells were electrophoretically characterized. Erythropoietin caused increased production of a haemoglobin type already present.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
