Hormonal regulation of mouse mammary tumor growth

Abstract

In view of reports that human breast cancer cells secrete growth factors that can replace estradiol in sustaining tumor growth [1], we have investigated whether hormone independent (HI) GR mouse mammary tumors can sustain growth of estrogen-depleted hormone dependent (HD) tumors. HD GR mammary tumor TS1 106 was grafted subcutaneously in the right flank of estrone plus progesterone treated castrated (020 × GR)F 1 mice. After 2 weeks the estrone treatment was stopped and the mice received 50,100 or 150 mg HI GR mammary tumor TS1 104 in the left flank. However, the regression of the HD tumor due to estrone depletion was not prevented or retarded by the HI grafts. In other experiments we investigated integrations of mouse mammary tumor virus (MMTV) proviral DNA in the DNA of GR mammary tumors. We could demonstrate the presence of two cell populations in tumor TS196, both HD but differing in MMTV DNA integration events. Our data indicate that exogenous integrations of MMTV proviruses can take place in mouse mammary tumor DNA without loss of hormone dependency of the tumors. Like in GR/Mtv-2 + mice, mammary tumor transplants differing in MMTV proviral integrations are also observed in 020/Mtv-2 + mice.