Hormonal regulation of adult sympathetic neurons: The effects of castration on neuropeptide Y, norepinephrine, and tyrosine hydroxylase activity

Abstract

Previous studies utilizing the hypogastric ganglia (HG) have indicated that gonadal steroids exert organizational and activational effects on noradrenergic biochemistry. Bilateral castration of male rodents at birth prevents the normal maturation of tyrosine hydroxylase (T-OH) activity in the HG; castration during adulthood results in a progressive decline in T-OH activity. Testosterone replacement corrects both the ontogenetic and adult functional alterations in the neurotransmitter-synthesizing enzyme. The present studies in adult male rats extend these previous observations and asked the question whether gonadal steroids regulate the neurotransmitters neuropeptide Y (NPY) and norepinephrine (NE) in the HG. Adult rodents were castrated and ganglia T-OH, NPY, and NE were examined at various time points after surgery. All three indices of sympathetic neuron biochemistry declined following castration, but they exhibited different profiles. It appears that hormones may affect enzyme activity and neurotransmitter pools differently within neurons. Testosterone replacement therapy fully restored T-OH activity, and NPY and NE levels in the HG. These studies extend the activational role of testosterone in regulating sympathetic neuron neurotransmitter and neuropeptide levels as well as neurotransmitter-synthesizing enzymes.