Hormonal measurements in late pregnancy and parturition in dairy cows—possible tools to monitor foetal well being

Abstract

Three dairy heifers (A, B and C) were induced to parturition with two prostaglandin (PG) F 2α injections on day 268 and 269 of pregnancy. Signs of approaching parturition were carefully observed. The following parameters were registered: degrees of calving difficulty, date and time of parturition, calf’s birth weight and calf’s sex. Body temperature was measured and blood samples were taken every 3 h 3 days before the first PGF 2α injection until 3 days after parturition. The plasma concentrations of the PGF 2α metabolite, progesterone, cortisol, oestrone sulphate and pregnancy associated glycoproteins (PAGs) were analysed. Heifers A, B and C delivered 48, 51 and 57 h after the first PGF 2α injection, respectively. Heifer A delivered without any signs of calving difficulty, whereas, the parturition was considered to be slight and moderate difficulty occurred in the delivery of heifers B and C, respectively. The calf of heifer C, without any abnormal gross-evidences, was stillborn. All animals had retained foetal membranes. A slight increase of the PGF 2α metabolite at the time of parturition was found only in heifer C, whereas the levels dramatically increased in all animals 15–24 h after parturition. At the same time, progesterone levels decreased within 3 h after the first PGF 2α injection ( P<0.05) and reached 0.8, 2.7 and 12.4 nmol/l at the time of parturition in heifers A, B and C, respectively. High release of cortisol at the time of parturition was seen in heifer C. Rising levels of oestrone sulphate around the time of parturition were recorded in all heifers, whereas, increasing levels of PAGs were recorded only in heifer A. In conclusion, the patterns of the PGF 2α metabolite, cortisol, progesterone and PAGs were changed in the cases of calving difficulty and stillbirth after PGF 2α-induction of parturition. However, the relationship between oestrone sulphate and PAGs and the status of foetal well being prior to parturition require further elucidation.