Abstract
Hormonal factors stimulating starch accumulation were extracted with water from the vascular tissue containing expiants of kale (Brassica oleracea L. var.medullosa cv. Krasa) and fractioned by elution chromatography on a column of polyvinylpyrrolidone. Their activities were compared with those of some known phytohormones and other metabolites using a special bioassay based on protein synthesis-dependent starch accumulation in stem pith expiants free of vascular tissue. The highest relative activities of the hormonal factors compared were as follows: Most active eluate fraction (14.0), cytokinins (8.0), auxins (2.3), ethylene precursor (2.0), ABA (0.8), 24-epi-brassinolide (0.7), GA3 (0.4), water control (1.0).
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