Abstract
Abstract Research was conducted in a three year old hop planting, Humulus lupulus Cluster (LI), in Mabton, WA on fine sandy loam. Pest presence was identified by using an aerial photograph and by digging up and examining the roots of 5 plants within the infested area. An average of 10 larvae per plant were found. A completely random design (3 plants per replicate) with 5 replicates was used. Treatments consisted of three entomopathogenic nematodes (Steinernema carpocapsae Weiser (All Strain), S. feltiae Filipjev (SN Strain), S. glaseri (Steiner) and water as a control. Nematodes were checked for viability under the microscope before application. Nematode species were applied on 27 May with a hand-operated soil injector (Marayuma 3MI, Tokyo, Japan) at three depths (10, 20 and 30 cm) and six locations at each depth around the base of the plant. Approximately 1.14 million nematodes (4.5 billion/acre) were injected in 180 ml of water per plant. One side of the plots was pre and the other side post irrigated for 24 h before/after nematode application by furrow irrigation. Plots were evaluated 5 weeks after treatments by digging up 60 plants and the surrounding soil (1 m diameter × 0.8 m depth) and counting P. decemlineata adults, pupae and larvae.
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