Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells.

R. Heru Prasetyo,Eka Pramyrtha Hestianah

doi:10.14202/vetworld.2017.711-715

R. Heru Prasetyo, Eka Pramyrtha Hestianah

Open Access

https://doi.org/10.14202/vetworld.2017.711-715

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Journal: Veterinary world	Publication Date: Jun 1, 2017
Citations: 13	License type: cc-by

Affiliation: Airlangga University

Abstract

Aim:This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells.Materials and Methods:Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.Results:There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.Conclusion:Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue

Highlights

Nutrition deficiency was an health problem, especially in development countries, include Indonesia
Data were collected from 32 male mices were divided into four treatments: Negative control group (T1) is normal liver without honey; positive control group (T2) is degenerative liver without honey; (T3) group is normal liver + 50% (v/v) honey in drinking water for 5 days; (T4) group is degenerative liver + 50% (v/v) honey in drinking water for 5 days
The results of the study are as follows: The effectively of honey was based on: (1) mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and vascular endothelial growth factor-1 (VEGF-1) expressions as regeneration Hsp70 as a marker of improvement [8] and VEGF-1 as a marker of growth factor [14]; and (3) regeneration histologically of liver tissue explain, why HSP70 and VEGF-1 are checked as markers

Summary

Introduction

Nutrition deficiency was an health problem, especially in development countries, include Indonesia. Since 2000 in Indonesia showed increased incidence of protein energy malnutrition (PEM) [1]. More than 50% of deaths of malnourished children due to lack of energy protein which increases the number of deaths due to intestinal disorders and diarrhea [1]. PEM is the most frequent cause of acquired immune deficiency [3] which can lead to opportunistic infection by intestinal parasites with symptoms of chronic diarrhea and that will increase mortality [4]. The mice were conditioned PEM in addition to obtained an increased expression of Hsp, decreased expression of prostaglandin E2, and expression of immunoglobulin A were examined immunohistochemically (IHC), histologically visible damage to the epithelium of the intestinal mucosa and occurs villus atrophy, which

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Conclusion