Abstract
Simultaneous addition of RNA preparations from two picornaviruses to facilitator compound resulted in greatly reduced numbers of plaques as compared to the number produced by similar concentrations of interferee RNA (that whose plaque formation was not blocked in interference experiments). A similar phenomenon was demonstrated with RNA from biochemical mutants of picornaviruses and their corresponding wild-type viruses. A method of biochemical blocking was shown to be as effective as that using antiserum. Infectivity of RNA preparations was markedly reduced by ultraviolet irradiation, heat, and snake venom phosphodiesterase; however, these agents did not significantly affect interfering capacity of the RNA preparations.
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