Homology modeling – a step towards vaccine development by analyzing structure of haemophilus influenza protein, transcriptional regulator H10994

Abstract

Introduction: Haemophilus influenza is a type of bacterium that is non motile, gram negative and causes poisoning and infection including pneumonia,bronchitis etc. In order to study the resistivity of H.influenza protein, transcription regulator: HI0433, homology modeling is an important step to predict structure. Material and methods: Bioinformatics such as CMR, BLAST, modeller Prcheck and Prosa was carried out to find 3D structure of protein. Results and Discussion: H.influenza has 1792 proteins. Out of these, 456 hypothetical proteins were found. Homology modeling of transcriptional regulator H10994 was done it consists of 8 helices and 7 beta sheets. Ramachandran plot has shown that it consists of 95.2% particles in maximum allowed regions, 2.9 % particles in fewer allowed region, 1.4% particles in inadequate allowed region, 5% particles in disallowed region. Conclusion: By homology modeling of H. influenza, transcriptional regulator protein (HI0433), structure was designed which has provided enough information for vaccine development to control its transcription for causing disease.