Abstract
A full-length cDNA encoding the 24-kDa flagellar Ca2+-binding protein (FCaBP) of the Dm28c clone ofTrypanosoma cruziwas cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of otherT. cruzistrains revealed greater than 97% sequence conservation. FCaBP-like genes are found inTrypanosoma conorhini, Trypanosoma freitasi, Trypanosoma lewisi, Herpetomonas megaseliae, Leptomonas seymouri,andPhytomonas serpens,but not inCrithidia deanei, Leishmania amazonensis,orEndotrypanum schaudinni. Among variousT. cruzistrains, FCaBP genes are located on chromosomes of different size, although all strains possess multiple FCaBP genes organized as tandemly arranged gene families. Northern and Western blot analyses revealed that FCaBP mRNAs are produced in all organisms possessing FCaBP-hybridizing sequences, indicating that expression of FCaBP or an FCaBP-like protein is common to a number of trypanosomatid species.
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