Abstract
Abstract Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.
Highlights
ObjectivesThe aims of this research were: (1) to assess the effect of GSH and/or homologous ram seminal plasma (HSP) supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim
Ram spermatozoa are highly susceptible to cryo-injury compared to other livestock species [1]
Following thawing (38 °С, 30 sec), straws were divided into four equal batches, its contents placed in glass tubes warmed at 37 °С and extended up to 50x106 cell/ml with one of the following extenders: Batch-1 was extended with control (C), Batch-2 with experimental-1 (E1) (C + GSH 5 mM), Batch-3 with experimental-2 (E2) (C + homologous ram seminal plasma (HSP) 20 %) and Batch-4 with experimental-3 (E3) (GSH 5 mM + HSP 20 %) CP-extenders, respectively
Summary
The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. The aims of this research were: (1) To assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility; and (2) To assess the effect of incubation in the context of the previous aim
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