Homologous nuclear-encoded mitochondrial and cytosolic isoproteins. A review of structure, biosynthesis and genes.

Abstract

Mitochondrial and cytosolic proteins may be expected to differ in specific traits due to their different intracellular location. However, the identification of these differences between mitochondrial and cytosolic proteins is complicated by the heterogeneity of the two protein groups. These difficulties have been overcome by comparing traits of homologous genes, which are derived from a common ancestor gene, and their gene products. An earlier report [Hartmann, C., Christen, P. & Jaussi, R. (1991) Nature 352, 762-763] describing a positive net charge difference between the mature parts of nuclear-encoded mitochondrial proteins and their homologous cytosolic isoproteins, could be corroborated by extending the data collection. New data were gathered from computer databases and published studies. The average isoelectric points of the mitochondrial and cytosolic isoproteins are 7.5 and 6.5, respectively. Depending on the type of protein, the observed difference results from differences in the number of basic and/or acidic amino acid residues in the isoproteins. Probably both the conditions required for mitochondrial protein import and the local conditions within the organelle furthered the evolution of basic protein structures. The contribution of the mitochondrial targeting peptide to the positive charge of precursors of nuclear-encoded mitochondrial proteins is largest when the value of the isoelectric point of the mature protein is small. This mutual dependence of the charge of the targeting peptide and the mature protein part supports the notion that positive charge is essential for mitochondrial protein import. Several traits other than electric charge, i.e. codon usage, chromosome location, structural organization or regulation of the genes, do not show specific differences between the sets of the heterotopic isoproteins. There is no preference of gene location for any of the gene sets; only rarely are the genes for a mitochondrial and a cytosolic isoprotein located on the same chromosome. A variant of the 3' splice-site consensus exists in genes of nuclear-encoded mitochondrial proteins. This is most likely a consequence of the evolution of the genes in separate lineages before endosymbiosis led to the formation of mitochondria. Some of the original mRNA group II intron self-splicing functions of the endosymbiont seem to persist in part of the cytosolic splicing machinery and apparently require a specific consensus sequence [Juretic, N., Jaussi, R., Mattes, U. & Christen, P. (1987) Nucleic Acids Res.15, 10083-10086].