Abstract
Abstract An assay technique, homogeneous enzyme immunoassay, is described for the quantitative determination of morphine derivatives in biological fluids. An enzyme is labeled with morphine. When the enzyme-labeled morphine is bound by antimorphine antibodies, the enzyme is rendered inactive. Free morphine competes with enzyme—morphine for antibody binding sites preventing inhibition of enzyme activity. Enzyme activity is thus directly related to the concentration of free morphine. See PDF for Equation Where the enzyme used is lysozyme, the assay can detect 0.5 µg of morphine per milliliter of urine with >95% confidence (CV, 5.0% at 0.5 µg/ml morphine). The technique gave nearly twice as many confirmed positives as did thin-layer chromatography in a comparative study of urines from a methadone maintenance program. The immediacy of the results (assay time: <1 min) permits use of the assay in hospital emergency rooms, law-enforcement facilities, and methadone treatment programs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
