Abstract
BackgroundAt present, six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions. Thus it is important to determine these sequences from additional strains in other countries, if possible, in order to clarify any anomalies regarding 16S rDNA sequence heterogeneity. Here, we clone and sequence the approximate full-length 16S rDNA from additional strains of T. equigenitalis isolated in Japan, Australia and France and compare these sequences to the existing published sequences.ResultsClarification of any anomalies regarding 16S rDNA sequence heterogeneity of T. equigenitalis was carried out. When cloning, sequencing and comparison of the approximate full-length 16S rDNA from 17 strains of T. equigenitalis isolated in Japan, Australia and France, nucleotide sequence differences were demonstrated at the six loci in the 1,469 nucleotide sequence. Moreover, 12 polymorphic sites occurred among 23 sequences of the 16S rDNA, including the six reference sequences.ConclusionHigh sequence similarity (99.5% or more) was observed throughout, except from nucleotide positions 138 to 501 where substitutions and deletions were noted.
Highlights
At present, six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions
Contagious equine metritis (CEM), which was first reported in thoroughbred mares during the 1977 breeding season in the UK [1], is an important bacterial genital infectious disease of horses caused by Taylorella equigenitalis
When Jang et al analyzed the phylogenetic position of T. asinigenitalis, which is a new second species within the genus Taylorella, and a gram-negative, nonmotile coccobacilli but phenotypically indistinguishable from T. equigenitalis, which was isolated from donkey jacks (Equus asinus) in the USA, in 1997 and 1998 [5], they described that T. equigenitalis appears to be homogeneous with respect to the 16S rDNA sequence, since all 15 strains, (11 American strains, two Dutch strains, one British and one Japanese isolate,) (Accession No AF297712 for a Dutch isolate 10783; AF297173 for an American isolate 96–178), together with the published sequence (X68645), were 100% identical [6]
Summary
Six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions. Contagious equine metritis (CEM), which was first reported in thoroughbred mares during the 1977 breeding season in the UK [1], is an important bacterial genital infectious disease of horses caused by Taylorella equigenitalis. When Jang et al analyzed the phylogenetic position of T. asinigenitalis, which is a new second species within the genus Taylorella, and a gram-negative, nonmotile coccobacilli but phenotypically indistinguishable from T. equigenitalis, which was isolated from donkey jacks (Equus asinus) in the USA, in 1997 and 1998 [5], they described that T. equigenitalis appears to be homogeneous with respect to the 16S rDNA sequence, since all 15 strains, (11 American strains, two Dutch strains, one British and one Japanese isolate,) (Accession No AF297712 for a Dutch isolate 10783; AF297173 for an American isolate 96–178), together with the published sequence (X68645), were 100% identical [6]
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