Abstract
Introduction: MiRNA expression alterations are closely related to growth, proliferation, and cell development. In addition, miR-187 has been found to regulate cellular proliferation. Further, spermatogenesis is a highly complicated process which is regulated through several genes targeted by miRNAs. However, no study has completely addressed the etiology of spermatogenic impairments. Accordingly, the present study examined the expression pattern of Homo sapiens (human) microRNA (hsa-mir-187) in the testicular biopsies of infertile men with non-obstructive azoospermia (NOA) and Sertoli cell-only syndrome (SCOS) subtype in comparison with those with obstructive azoospermia (OA). Methods: Quantitative reverse‐transcription polymerase chain reaction analysis was performed to assess the expression level of hsa-mir-187 using the ΔΔCt method. Results: Our data indicated that hsa-mir-187 was significantly up-regulated in the testis of the SCOS group (P˂ 0.05) in comparison with the OA group. Conclusion: In general, the findings suggest that the differential expression of hsa-mir-187 is linked with male infertility and shed more light on molecular defects that underlie spermatogenic impairments.
Highlights
MiRNA expression alterations are closely related to growth, proliferation, and cell development
In general, the findings suggest that the differential expression of hsa-mir-187 is linked with male infertility and shed more light on molecular defects that underlie spermatogenic impairments
Infertile men participating in this study were classified as non-obstructive azoospermia (NOA) (SCOS) and obstructive azoospermia (OA) based on histological analysis
Summary
Infertility, as a worldwide reproductive health problem, is estimated to affect nearly 15% of couples.[1]. Azoospermia, which is referred to the lack of sperms in the ejaculate, comprises a remarkable portion of idiopathic male infertility cases and is caused by either primary testicular failure (non-obstructive azoospermia, NOA) or sperm blockage from entering the semen (obstructive azoospermia, OA). Several genomewide expression studies have focused on identifying testicular dysregulated miRNAs in infertile men.[6,7,8] It is strongly suggested that hsa-miR-187, as a tumor suppressor, Piryaei et al is critically involved in regulating cellular proliferation.[9] the role of miR-187 in male infertility pathogenesis has not been understood yet. A better understanding of the changes in miRNA expression in patients with impaired spermatogenesis might pave the way to propose new attractive therapeutic targets. The present study aimed to examine the expression pattern of hsa-miR-187-3p in the testis of OA and idiopathic NOA infertile patients. It is hoped that our study increases the understanding of the molecular role of miR-187 in impaired spermatogenesis fertility
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