Abstract
Rayleigh-Sommerfeld back-propagation can be used to reconstruct the three-dimensional light field responsible for the recorded intensity in an in-line hologram. Deconvolving the volumetric reconstruction with an optimal kernel derived from the Rayleigh-Sommerfeld propagator itself emphasizes the objects responsible for the scattering pattern while suppressing both the propagating light and also such artifacts as the twin image. Bright features in the deconvolved volume may be identified with such objects as colloidal spheres and nanorods. Tracking their thermally-driven Brownian motion through multiple holographic video images provides estimates of the tracking resolution, which approaches 1 nm in all three dimensions.
Highlights
“Rotational and translational diffusion of copper oxide nanorods measured with holographic video microscopy,” Opt
In-line holographic video microscopy offers time-resolved information regarding the threedimensional distribution of matter that scatters a beam of laser light [1, 2]
Dynamical measurements based on tracking such features through holographic video sequences have demonstrated 10 nm resolution for three-dimensional location of colloidal spheres [5] and nanorods [11], albeit with large systematic axial offsets in some cases [5]
Summary
G. Grier, “Characterizing and tracking single colloidal particles with video holographic microscopy,” Opt. Express 15, 18,275–18,282 (2007). G. Grier, “Strategies for three-dimensional particle tracking with holographic video microscopy,” Opt. Express 18, 13,563–13,573 (2010). G. Grier, “Rotational and translational diffusion of copper oxide nanorods measured with holographic video microscopy,” Opt. Express 18, 6555–6562 (2010).
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