Hollow fiber–stir bar sorptive extraction and microwave assisted derivatization of amino acids in biological matrices

Abstract

A kind of solid phase microextraction configuration combining the principles of hollow fiber solid phase microextraction (HF–SPME) and stir bar sorptive extraction (SBSE) is presented. The main feature of HF–SBSE is the use of microporous hollow fiber acting as the carrier and filter, while a thin stainless steel wire and silica microspheres in the lumen of hollow fiber respectively acting as the magnetic stirrer and the dispersed sorbents for the collection and extraction of the target analytes, thus affording extraction process like SBSE. Moreover, the prepared hollow fiber stir bar was applied to direct microextraction and microwave assisted derivatization with N,O–Bis(trimethylsilyl)trifluroacetamide (BSTFA) of four amino acids in rats’ urine and cerebrospinal fluid followed by gas chromatography mass spectrometric analysis. The limits of detection for four amino acids were found to be in the range of 0.0003–0.017μgmL−1, and all the analytes did not exhibit any lack of fit. The extraction recoveries using HF–SBSE techniques ranged from 71.8% to 102.3%. The results indicated that hollow fiber stir bar sorptive extraction was a promising technique for the enrichment and direct derivatization of analytes extracted from biological matrices without sample clean–up.