Abstract
This study aimed to investigate the effects of Ser/Cys polymorphism in hOGG1 gene, Arg/Pro polymorphism in p53 gene, smoking and their interactions on the development of lung cancer. Ser/Cys polymorphism in hOGG1 and Arg/Pro polymorphism in p53 among 124 patients with lung cancer and 128 normal people were detected using PCR-RFLP. At the same time, smoking status was investigated between the two groups. Logistic regression was used to estimate the effects of Ser/Cys polymorphism and Arg/Pro polymorphisms, smoking and their interactions on the development of lung cancer. ORs (95% CI) of smoking, hOGG1 Cys/Cys and p53 Pro/ Pro genotypes were 2.34 (1.41-3.88), 2.12 (1.03-4.39), and 2.12 (1.15-3.94), respectively. The interaction model of smoking and Cys/Cys was super-multiplicative or multiplicative, and the OR (95% CI) for their interaction item was 1.67 (0.36 -7.78). The interaction model of smoking and Pro/Pro was super-multiplicative with an OR (95%CI) of their interaction item of 5.03 (1.26-20.1). The interaction model of Pro/Pro and Cys/Cys was multiplicative and the OR (95%CI) of their interaction item was 0.99 (0.19-5.28). Smoking, hOGG1 Cys/Cys, p53 Pro/Pro and their interactions may be the important factors leading to the development of lung cancer.
Highlights
Lung cancer is one of the most common malignant tumors, and it is one of the key foci of cancer research in the world
Apart from the definite role played by smoking in leading to lung cancer, the role played by hereditary susceptibility has attracted more and more attention
The products were detected by 2% agarose gel electrophoresis, and the results showed that specific bands of 505bp were obtained, which were consistent with those expected
Summary
Lung cancer is one of the most common malignant tumors, and it is one of the key foci of cancer research in the world. Studies mainly focus on gene polymorphisms of carcinogen-metabolizing enzymes and DNA repair enzymes. DNA damage and repair play an important role in the process of cellular canceration. A low DNA repair ability caused by polymorphism of the DNA repair-related gene may be an important factor determining the hereditary susceptibility to lung cancer. Cells can repair 8-OH-G via the base excision repair channel, and if the repair can’t be done, cellular apoptosis avoid the mutations which may lead to the initiation of tumors. Polymorphisms of these repair-related genes may affect the repair ability, which, in turn, affect the susceptibility to lung tumor
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
