Abstract

Perisplenic nodes and splenic tumor nodules were obtained from two patients with Hodgkin's disease and used to immunize individually assigned rabbits. After immunization, the antiserums were absorbed with the normal splenic tissue from the same patient. The absorbed antiserums were used to identify a tumor-associated antigen by indirect immunofluorescence in cryostat sections. Tumor nodules fluoresced, whereas adjacent normal splenic tissue, serving as concomitant control, did not. Common antigenicity between patients was also demonstrated by fluorescence of absorbed antiserums cross-reactive with cryostat sections of other patients' tumors.

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