Abstract
Aurora-A is a serine/threonine kinase that involved in centrosome maturation, mitotic entry, spindle assembly and chromosome segregation. Dysregulation of Aurora-A results in centrosome amplification, cytokinesis failure, aneuploidy, and finally leads to tumor formation. It was reported that Aurora-A is overexpressed in a lot of cancers. Our previously studies indicated that EGF can translational up-regulate the expression of Aurora-A through a specific 5’-untranslated region (5’UTR). In addition, hnRNP Q1 and hnRNP A2/B1 may participate the translational regulation of Aurora-A mRNA. In this study, we investigate the role of hnRNP Q1 and hnRNP A2/B1 in translational regulating Aurora-A. The expression pattern of Aurora-A mRNA isoforms in human colorectal cancer was firstly confirmed by real-time PCR. In vivo translation assay and ribosomal protein S6-immunoprecipitation assay showed that hnRNP Q1 can increase the translation efficiency of Aurora-A, however hnRNP A2/B1 plays an opposite role. The biotin pull-down assay indicates hnRNP Q1 and hnRNP A2/B1 prefers to interact with different region of Aurora-A 5’UTR. BIAcore assay confirmed the Aurora-A 5’UTR-binding domain of hnRNP Q1. The expression of Aurora-A and hnRNP Q presents a positive correlation in clinical human colorectal cancer specimens by RT-PCR analysis. Taken together, our results suggest that hnNRP Q1 and hnRNP A2/B1 may translationally regulate Aurora-A mRNA through its 5’UTR.
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