Abstract
Oral squamous cell carcinoma (OSCC) is the leading cause of death related to oral diseases. The mechanisms of OSCC development remain largely unknown. Heterogeneous nuclear ribonucleoprotein L (HnRNP L) is a multi-functional splicing factor. It has been reported to be an important regulator of apoptosis. However, the functions of hnRNP L in cancer need to be further explored. In the present study, we found that OSCC tissues expressed significantly higher levels of hnRNP L than normal tissues. Depletion of hnRNP L retarded cell growth, cell migration, and tumorigenesis of OSCC cells. HnRNP L regulates both the expression of oncogenic splicing factor SRSF3 and the alternative splicing of SRSF3 exon 4. Expression of hnRNP L is correlated with SRSF3 expression in OSCC tissues. These findings suggest that hnRNP L is important for the pathogenesis of OSCC and may be a novel potential therapeutic target of OSCC.
Highlights
HnRNP L is a multifunctional splicing factor
We found that hnRNP L is significantly overexpressed in Oral squamous cell carcinoma (OSCC) tissues compared with normal oral mucosal tissues
We found that knockdown of hnRNP L increased the cleavage of Poly (ADP-ribose) polymerase-1 (PARP) by more than two-fold (Fig. 2B) compared with control, indicating a slightly increased apoptosis induced by hnRNP L knockdown
Summary
HnRNP L is a multifunctional splicing factor. HnRNP L has been reported to be involved in tumorigenesis. Goehe et al.[17] found that down-regulation of hnRNP L significantly affected tumorigenic capacity in a non-small cell lung cancer cell line via apoptosis. A proteomics study showed that the expression level of hnRNP L in esophageal cancer cell line is over five-fold higher than that in an immortal cell line[18]. We found that hnRNP L is significantly overexpressed in OSCC tissues compared with normal oral mucosal tissues. HnRNP L is important for OSCC cell growth, cell migration, and tumorigenesis. Oncogenic splicing factor SRSF3 is a novel target of hnRNP L. Our results uncovered new characteristics of hnRNP L in tumorigenesis and its essential target
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