HMGB1 in an experimental model of acute lung injury.

Abstract

Event Abstract Back to Event HMGB1 in an experimental model of acute lung injury. Cilmery S. Kurokawa1*, José R. Fioretto1, João P. Araujo2, Rafaelle B. Pires1, Marcos A. Moraes1, Rossano C. Bonatto1 and Mario F. Carpi1 1 Botucatu Medical School - São Paulo State University - UNESP, Brazil 2 Bioscience Institute of Botucatu - UNESP, Microbiology and Immunology, Brazil Despite recent therapeutic advances in acute respiratory distress syndrome (ARDS), its physiological and immunological aspects have not been completely understood. Recently, HMGB1, a nonhistone, chromatin-associated protein and a cytokine produced by macrophages, has been associated with sustained inflammatory response after injury or infection of tissue and lung injury. The aim of the study was to measure HMGB1 levels in rabbits with acute lung injury (ALI) induced by tracheal infusion of warm saline. Methods: Thirty rabbits were instrumented and randomly assigned into two groups: 1) animals with no ALI (Control Group-CG; n = 15); 2) animals with severe ALI (SG Group; n = 15). The groups were ventilated during 4 hours with protective conventional mechanical ventilation (CMV). After ventilation, HMGB1 levels from bronchoalveolar lavage fluid (BAL) and plasma were measured as well as the percentage of white cell from BAL. Left lung was collected for histopathology analysis. Results:Lung injury decreased pulmonary compliance (SG before: 1.86±0.576 > SG after: 0.67± 0.24) and oxygenation (PaO2 - SG before: 427.92 ± 89.90 > SG after: 68.18 ± 19.08). Percentage of neutrophil (p=0.001) and lung injury score were higher in SG than CG. Higher levels of HMGB1 in BAL were found in SG as compared to CG group [SG: 62 (20.30-79.41) > CG: 15.60 (13.86-25.81); p<0.035]. No statistically significant difference was found in HMGB1 plasmatic levels when groups were compared. Conclusions:HMGB1 is released earlier in alveolar space in acute lung lesion induced by warm saline and can contribute to the pathogenesis of ALI. Fapesp grants:2011/15144-2 Acknowledgements This study was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo);grants 2011Q15144-2. We would like to thank Clinical and Experimental Research Center of Pediatrics Department Keywords: HMGB1, lung lesion, rabbit, Lung Diseases, Cytokines Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Kurokawa CS, Fioretto JR, Araujo JP, Pires RB, Moraes MA, Bonatto RC and Carpi MF (2013). HMGB1 in an experimental model of acute lung injury.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00525 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 31 May 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Cilmery S Kurokawa, Botucatu Medical School - São Paulo State University - UNESP, Botucatu, Sao Paulo, 18618970, Brazil, cskurokawa@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Cilmery S Kurokawa José R Fioretto João P Araujo Rafaelle B Pires Marcos A Moraes Rossano C Bonatto Mario F Carpi Google Cilmery S Kurokawa José R Fioretto João P Araujo Rafaelle B Pires Marcos A Moraes Rossano C Bonatto Mario F Carpi Google Scholar Cilmery S Kurokawa José R Fioretto João P Araujo Rafaelle B Pires Marcos A Moraes Rossano C Bonatto Mario F Carpi PubMed Cilmery S Kurokawa José R Fioretto João P Araujo Rafaelle B Pires Marcos A Moraes Rossano C Bonatto Mario F Carpi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.