HL-A typing of kidney cells and lymphocytes.

Abstract

Abstract. Fresh human kidney cells were typed for HL‐A antigens by means of the micro‐immune adherence assay (MIAA). Lymphocytes from the same individuals were typed both by means of the MIAA and the microcytotoxicity test. Difference in typing results between the two types of cells was observed in 10% of the cases. When cultured kidney cells were used for HL‐A typing, a greater difference was found, namely 28%. This can be explained by the poor reproducibility of the MIAA if performed on cultured kidney cells.In 7% of all cases, an HL‐A factor was found on kidney cells which could not be demonstrated on the lymphocytes. This may be explained by the fact that the MIAA of fresh kidney cells is more sensitive for detecting HL‐A antigens than the MIAA or the microcytotoxicity test of lymphocytes. Lymphocytes not reacting in the MIAA were able to absorb antibodies from the anti‐HL‐A sera that showed a positive reaction in the MIAA with fresh kidney cells from the same individuals. In one case, absorption of antibodies by lymphocytes could not be demonstrated. Kidney‐specific antibodies could not be detected in anti‐HL‐A sera, nor could differences in typing results between the two types of cells be explained by the presence of blood‐group antibodies.