HLA-DQ2 and -DQ8 genotype frequency in Syrian celiac disease children: HLA-DQ relative risks evaluation

Issam Khansaa,Batoul Jazairi,Hossam Murad,Lina Khouri,Doaa Olabi

doi:10.1186/s12876-018-0802-2

Issam Khansaa, Batoul Jazairi + Show 3 more

Open Access

https://doi.org/10.1186/s12876-018-0802-2

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Abstract

BackgroundCeliac disease (CD) is a common autoimmune disease in Syria which manifesting with inflammation of the small intestine and with various extra intestinal symptoms. The disease is associated with human HLA-DQ genes encoding HLA-DQ2 and DQ8 proteins.MethodsIn this study, 49 children patients of CD and 58 healthy control samples were genotyped for HLA-DQ genes using SSP-PCR technique. Relative risks for different genotypes were also evaluated.ResultsThe DQB1*0201 allele was the most common in the patients (77.6%) followed by DQB1*0302 allele (10.2%). The highest HLA-DQB risk for CD development was found in patients carriers a DQ2.5/DQ8 genotype (1/10), followed by the patients carriers DQ2.5/DQ2.5 (1/12).ConclusionThe significant differences in the frequency of HLA-DQ2 and HLA-DQ8 in Syrian patients in compared with controls and relative risks predicted demonstrated the importance role of these alleles in the development of CD in Syrian children patients.

Highlights

Celiac disease (CD) is a common autoimmune disease in Syria which manifesting with inflammation of the small intestine and with various extra intestinal symptoms
In Syria, previous reports indicated that Syrian population has high prevalence of the CD (1/62) in healthy blood donors [16, 17], but no published study about the link of Human leukocyte antigen (HLA)-DQ alleles and Syrian celiac patients; so, we aim to study the distribution of the CD genes: DQ2 and DQ8 in 49 children patients in comparable with 58 control samples of the same ages
HLA-DQB1 genotype was performed by sequence-specific primer-polymerase chain reaction (SSP-PCR) method using published primers [20] and for HLA-DQA1 genotype [21]

Summary

Introduction

Celiac disease (CD) is a common autoimmune disease in Syria which manifesting with inflammation of the small intestine and with various extra intestinal symptoms. Celiac disease is an immune-mediated disorder with a strong genetic predisposition and dietary gluten as an environmental trigger [1]. This disease causes inflammation in the small intestinal mucosa. Several ways to diagnosis celiac disease using anti-tissue transglutaminase (anti-TG2) test, anti-endomysium (EMA) auto-antibodies and small bowel biopsy [3]. This disease is widespread in most Mediterranean countries [4], and it estimated that about more than 5 million patients will be affected in the 10 years in the Mediterranean region [5].

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