HLA analysis of Mexican candidates for bone marrow transplantation and probability of finding compatible related donors

Abstract

IntroductionOncohematological disorders are the main cause of morbidity in the Mexican population from 1 to 19 years old, where megakaryoblastic and promyelocitic leukemias are more frequent. Considering that the success of a transplant is multifactorial, the criterion of compatibility in the HLA system is crucial and even more so when the source of HSC is bone marrow. ObjectiveTo determine the frequency of the HLA genotype in Mexican candidates who require a bone marrow transplant from related donors and the probability to find donors. Materials and methodsOne hundred twenty-six candidates for bone marrow transplant and related donors were tested for HLA class I (–A*, −B* alleles) and class II (–DRB1* allele) in intermediate–resolution, as the first phase in the choice of the possible donor. The criteria to identify donors were determined by antigen–matched in each HLA haplotype as follows: 4/6, 5/6 and 6/6 at the HLA–A*, HLA–B*, and HLA–DRB1* alleles. ResultsOf all the candidates analyzed, 57.93%, at least one bone marrow donor was identified; in 53 cases, no donor was found. The average size of the families was 4.79 ± 1.06 members. A higher percentage of compatibility with grade 6/6 (31.6%) was identified with brothers, followed by sisters in 25.3%. The probability to find at least one compatible potential donor was 1.51 ± 0.92 donors. ConclusionIn the first phase to select donors, Mexican patients studied in this work, have a compatible donor, however the grade of resolution test influenced in the probability identified.